Microcantilever operated in liquid environment for in-vitro biomolecular detection
We have recently reported the piezoelectric thick film microcantilever, which enables the in-situ real-time detection of the protein related to disease (e.g. C reactive protein) in liquid environment. This work was published at APL (click here).
"In-situ real-time monitoring of biomolecular interactions based on resonating microcantilevers immersed in a viscous fluid"
We report the precise (noise-free) in-situ real-time monitoring of a specific protein antigen-antibody interaction by using a resonating microcantilever immersed in a viscous fluid. In this work, we utilized a resonating piezoelectric thick film microcantilever, which exhibits the high quality factor (e.g. Q = 15) in a viscous liquid at a viscosity comparable to that of human blood serum. This implies a great potential of our resonating microcantilever to in-situ biosensor applications. It is shown that our microcantilever enables us to monitor the C reactive protein (CRP) antigen-antibody interactions in real-time, providing an insight into the protein binding kinetics.