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Deformation of the cell nucleus under indentation: Mechanics and Mechanisms

Ashkan Vaziri's picture

Computational models of the cell nucleus, along with experimental observations, can help in understanding the biomechanics of force-induced nuclear deformation and mechanisms of stress transition throughout the nucleus. Here, we develop a computational model for an isolated nucleus undergoing indentation, which includes separate components representing the nucleoplasm and the nuclear envelope. The nuclear envelope itself is composed of three separate layers: two thin elastic layers representing the inner and outer nuclear membranes and one thicker layer representing the nuclear lamina. The proposed model is capable of separating the structural role of major nuclear components in the force-induced biological response of the nucleus (and ultimately the cell). A systematic analysis is carried out to explore the role of major individual nuclear elements, namely inner and outer membranes, nuclear lamina, and nucleoplasm, as well as the loading and experimental factors such as indentation rate and probe angle, on the biomechanical response of an isolated nucleus in atomic force microscopy indentation experiment. The paper can be accessed here


Xi Chen's picture

We study the indentation and mechanics of cells/proteins a lot, and would like to learn something from your paper.

Ashkan Vaziri's picture

Thanks for your interest. I have included a link to the paper.

On Page 2127 of your article, it is stated:

"It is noteworthy that the current data on theapparent stiffness of cell nuclei are rather divergent, withvalues ranging from 18 Pa to nearly 10 kPa12,25–27 due tofactors such as cell type, measurement techniques and

conditions, length scale of interest, and also interpretation methods."

 How is apparent stiffness is defined here? Note I am new to the field.   



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