A double cantilever beam (DCB)
specimen is created by affixing the two halves together using a bi-layer of
PC-3 prostate cancer cells. The specimen is pinned at a bottom corner, and the
upper corner on the same end is displaced with the force-displacement profile
being recorded. This upper corner is displaced until the crack, a portion of
the specimen where cell grown has been selectively inhibited, propagates
through the cell layer. The critical value of force at which the crack
propagates through the cell layer is used, in conjunction with the initial
crack length, to determine the toughness via the compliance-energy method (Ripling, et al. 1971). A method for performing a FE analysis of the