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Micromechanical observation of the kinetics of biomolecular interactions in liquid

We have recently suggested the potential of resonant microcantilever for quantitative study on the kinetics of biomolecular interactions such as protein-protein interaction and DNA hybridization. We have employed the Langmuir kinetic model for describing the molecular interactions on the surface, which leads to change of overall mass of cantilever responsible for resonant frequency shift. Such Langmuir kinetic model dictates the in situ resonant frequency shift due to biomolecular interaction in liquid. This indicates that resonant cantilever is able to provide the information of biochemical reaction. This work was published in Applied Physics Letters in Oct 31, 2008.

ABSTRACT
Resonant microcantilevers have recently enabled the label-free detection of biomolecules. Here, we observed the kinetics of biomolecular interactions such as antigen-antibody interactions and/or DNA hybridization based on a resonant frequency shift, obeying Langmuir kinetics, measured in a buffer solution. It is shown that the kinetics of DNA adsorptions on the surface is governed by intermolecular interactions between adsorbed DNA molecules. It is also shown that the kinetics of DNA hybridization is determined by the intermolecular interaction. It is implied that resonant microcantilever in buffer solution may allow for gaining insights into the kinetics of various molecular interactions. [link: http://link.aip.org/link/?APPLAB/93/173901/1]

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