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lack of sensitivity

Submitted by adelajda on

Hi everybody.  My name is Ada. I am a new user on this forum.

I am working with microcantilevers (MCs). Recently I experienced lack of sensitivity. I use  MCs coated with nanostructured gold and I use SAM to attach different biological molecules e.g. Protein A, IgG, antiIgG. Everything was working fine until recently.

Would enybody have any sugestions what might be wrong?

Thanks,

Ada