User login


You are here

Force response and actin remodeling (agglomeration) in fibroblasts due to lateral indentation

We report the loading and unloading force response of single living adherent fibroblasts due to large lateral indentation obtained by a two-component microelectromechanical systems (MEMS) force sensor. Strong hysteretic force response is observed for all the tested cells. For the loading process, the force response is linear (often with small initial non-linearity) to a deformation scale comparable to the undeformed cell size, followed by plastic yielding. In situ visualization of actin fibers (GFP) reveals that during the indentation process, actin network depolymerizes irreversibly at discrete locations to form well-defined circular actin agglomerates all over the cell, which explains the irreversibility of the force response. Similar agglomeration is observed when the cell is compressed laterally by a micro plate. The distribution pattern of the agglomerates strongly correlates with the arrangement of the actin fibers of the pre-indented cell. The size of the agglomerates increases with time as ta  with a= 2~3 initially,   followed by a=.5~1. The higher growth rate suggests influx of actin into the agglomerates. The slower rate suggests a diffusive spreading, but the diffusion constant is two orders of magnitude lower than that of an actin monomer through the cytoplasm. Actin agglomeration has previously been observed due to biochemical treatment, gamma-radiation, and ischemic injury, and has been identified as a precursor to cell death. We believe, this is the first evidence of actin agglomeration due to mechanical stimuli. The study demonstrates that living cells may initiate similar functionalities in response to dissimilar mechanical and biochemical stimuli.


To appear in Acta Biomaterialia 3 (2007) 77-87. 

Image icon Cell1.jpg853.24 KB
PDF icon Yang and Saif.pdf1.01 MB
Image icon Cell2.jpg142.65 KB


Interesting opportunities have arisen in the past to carry out biochemical studies on cultures of fibroblasts from biopsies taken from humans. Although fibroblasts were thought to be cells in which many specialised cell functions are not expressed, studies carried out on the fibroblasts taken from patients with Lesch-Nyhan Syndrome, mucopolysaccharide accumulation disorders or inherited hypercholesterolaemia have clearly shown their importance in solving particular biochemical problems. In vitro studies have shown the development of the specialised structure and function of human dermal fibroblasts. The size of the cells after division and of the nuclei increases dramatically during this process of instant differentiation.

Subscribe to Comments for "Force response and actin remodeling (agglomeration) in fibroblasts due to lateral indentation"

Recent comments

More comments


Subscribe to Syndicate