Mechanics of microtubule buckling in living cells

As the most rigid cytoskeletal filaments, microtubules bear compressive forces in living cells, balancing the tensile forces within the cytoskeleton to maintain the cell shape. It is often observed that, in living cells, microtubules under compression severely buckle into short wavelengths. By contrast, when compressed, isolated microtubules in vitro buckle into single long-wavelength arcs. The critical buckling force of the microtubules in vitro is two orders of magnitude lower than that of the microtubules in living cells.

To explain this discrepancy, we describe a mechanics model of microtubule buckling in living cells. The model investigates the effect of the surrounding filament network and the cytosol on the microtubule buckling. The results show that, while the buckling wavelength is set by the interplay between the microtubules and the elastic surrounding filament network, the buckling growth rate is set by the viscous cytosol. The model quantitatively correlates the microtubule bending rigidity, the surrounding filament network elasticity, and the cytosol viscosity with the buckling wavelength, the buckling growth rate, and the buckling amplitude of the microtubules. Such results shed light on designing a unified experimental protocol to measure various critical mechanical properties of subcellular structures in living cells.

The paper is available at: 

Li, T., A mechanics model of microtubule buckling in living cells. Journal of Biomechanics, 41, 1722-1729 (2008), doi:10.1016/j.jbiomech.2008.03.003