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Non linear cell mechanics

Submitted by Daniel Isabey on

Ex-vivo measure of stress-strain relationships in populations of living adherent cells by means of ligand-coated ferromagnetic microbeads (mean diameter: 4.5 µm) attached to the transmembrane mechanoreceptors which are linked to the cytoskeleton (CSK), reveal non linear cell mechanical behavior. However, this non linear cell mechanical behaviour is subjected to controversy for various reasons. First, it has not been systematically found. Results seem to depend on the micromanipulation method used and/or the cell type.

tension of cu film on Pi substrate

Submitted by Zaiwang Huang on
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Dear professor suo I am a master graduate of professor sun jun in xi'an jiaotong university, I have done some research on the tension of cu film on Pi substrate. I have a question about the mechanical behavior of thin film:the range of elastic deformation is enlarged just as the plastic stage in your simulation results, since the mutiple neckings result in improved plasticity of Pi-bonded Cu film.could you give me some advice? many thanks

Effect of surface energy on the yield strength of nanoporous materials

Submitted by Weixu Zhang on

This is a very rough manuscript but including the original material we used. Any criticism or suggestion is welcome. The only aim of this letter is to reflect the multi-effect of surface energy on material or structure in nanosize scale. Here we report the effect of surface energy on the yield strength of nanoporous materials. The conventional micromechanics method is extended to consider the surface effect and expression of effective yield surface of nanoporous materials in complex stress state is derived.

TWELVE STEPS TO A WINNING RESEARCH PROPOSAL

Submitted by Nanshu Lu on

By George A. Hazelrigg, National Science Foundation

I have been an NSF program director for 18 years. During this time, I have personally administered the review of some 3,000 proposals and been involved in the review of perhaps another 10,000. Through this experience, I have come to see that often there are real differences between winning proposals and losing proposals. The differences are clear. Largely, they are not subjective differences or differences of quality; to a large extent, losing proposals are just plain missing elements that are found in winning proposals. Although I have known this for some time, a recent experience reinforced it.

Intracellular CalciumWaves in Bone Cell Networks Under Single Cell Nanoindentation

Submitted by Ed Guo on

In this study, bone cells were successfully cultured into a micropatterned network with dimensions close to that of in vivo osteocyte networks using microcontact printing and self-assembled monolyers (SAMs). The optimal geometric parameters for the formation of these networks were determined in terms of circle diameters and line widths. Bone cells patterned in these networks were also able to form gap junctions with each other, shown by immunofluorescent staining for the gap junction protein connexin 43, as well as the transfer of gap-junction permeable calcein-AM dye.

No need to worry about gravity at the atomic-/nano-scale

Submitted by Zhenyu Zhang on

When a metal is grown onto a substrate of itself (homoepitaxy), the growth front is typically smooth, or at most is roughened by the formation of shallow hills (called surface mounds). The underlying reason for the roughening has been recognized to be of kinetic nature: Atoms landed on an upper terrace do not have enough time to overcome the "road blocks" provided by the steps and fill all the valleys (known as the Villian instability).

Micro cantilever pre-stress

Submitted by Iskandar Samad on
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Dear all,

Im a PhD student in Cambridge Uni, UK working in the field of MEMS, and as part of my work, of late Ive been looking at deriving materials properties of MEMS thin film materials by means of resonant testing. The basic outline of the experiment is first creating free standing rectangular cantilevers of the material under test, evaporate with gold to increase reflectivity (when needed), then (under reasonable vacuum) applying a base excitation using a chirp signal into a piezo actuator and logging the cantilever tip response using a laser/photodetector setup. The frequency response is then calculated and the modal frequencies noted.

To determine the materials' properties, both an analytical model (with bending/torsion modes) and finite element model (using 2D mindlin) are created with similar geometry as the sample, and by minimising the squared relative error between the measured modes and those from the models, the value of Young's modulus (known density) and poissons ratio may be determined iteratively. These yield fairly consistent results.

To take the work further I now feel I should also include the effects of residual stress in the cantilevers. The method Ive been looking at is by using finite element (via COMSOL) - the beam geometry is created and loaded with the stress model ('surface stress' as a force tangential to the top boundary, and gradient stress as a 'tangential' force that varies from +F to -F from top to bottom boundary of the cantilever). The model is solved statically, and the deformed shape is then saved as the linearisation point for the next model, which then computes the eigenfrequencies. Btw I can only do this in 3D FE, which makes computation times quite long hence using iteration to quantify this stress highly unlikely.

In any case, is there an analytical model I can use to model the effect of this stress on multiple modes of a cantilever. Id like to verify whether the FE is giving me anything close to ball park numbers before I work out a means to compare them with experimental results. I was thinking of using the Rayleigh method by representing the effect of prestress as an additional term in the potential energy. The original mode shapes, with some modification will be used to evaluate the two energy integrals. The potential energy due to stress is worked out by measuring the static deflected shape using a zygo inteferometer - some rough model is used, with the beam curvature and peak deflection as input to work out the amount of this energy. Not having much experience in mechanics (i was an electronics undergrad!), Im not sure how good an estimate this would be, if at all its a useable or even possible one. Will the extra energy factor in to the torsion modes just the same?